taptonab2016-poster.pdf |
london_2015_poster_corrected.pdf |
Wellcome Poster 2015 Abstract
The cyclin dependent protein kinase family regulates a wide range of cellular functions such as cell cycle progression, differentiation, and apoptosis. They are kinase enzymes which regulate other proteins by attaching a phosphate to serine or threonine amino acid residues in target proteins. They have two functional domains, a cyclin binding domain and a kinase domain. Cyclin binding activates these proteins mediating a cellular process via a signalling cascade. CDKL1 has been classed as cyclin dependent “like” as it contains the conserved domain for binding cyclin “KKIARLE” as well as a kinase domain. The structure of the kinase domain has been determined and is very similar to the well characterised human CDK2 (1,2). No function has been assigned to CDKL1. Our study identified an elevated expression in response to heart attack and In Situ hybridisation analysis of Zebrafish indicates that CDKL1 may signal blood vessel formation. Through Genome Expression Omnibus searches we propose that CDKL1 mediates this through the Sonic Hedgehog, VEGF, NOTCH signalling pathway.
The cyclin dependent protein kinase family regulates a wide range of cellular functions such as cell cycle progression, differentiation, and apoptosis. They are kinase enzymes which regulate other proteins by attaching a phosphate to serine or threonine amino acid residues in target proteins. They have two functional domains, a cyclin binding domain and a kinase domain. Cyclin binding activates these proteins mediating a cellular process via a signalling cascade. CDKL1 has been classed as cyclin dependent “like” as it contains the conserved domain for binding cyclin “KKIARLE” as well as a kinase domain. The structure of the kinase domain has been determined and is very similar to the well characterised human CDK2 (1,2). No function has been assigned to CDKL1. Our study identified an elevated expression in response to heart attack and In Situ hybridisation analysis of Zebrafish indicates that CDKL1 may signal blood vessel formation. Through Genome Expression Omnibus searches we propose that CDKL1 mediates this through the Sonic Hedgehog, VEGF, NOTCH signalling pathway.
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Wellcome Poster 2014 Abstract
Cardiovascular disease (CVD) is the single largest cause of death in the UK. It accounts for around 200,000 deaths a year, mostly as a result of heart attack or stroke. Arterial blockage (or occlusion) is a major factor. We aim to characterise novel genes that are differentially expressed in a clinical analysis of patients who have suffered a heart attack by studying orthologous genes in the Zebrafish cardiovascular system. These genes may be important in the response to a heart attack or act as biological markers for heart disease. We analysed a list of 82 genes and selected ALAD, CDKL1, FECH, and TBC1D19 for further study. Preliminary data for Cyclin-Dependent Kinase-Like 1 (CDKL1) show that in 24-48hpf embryos it is localised to the hypochord, a precursor to the dorsal aorta. Its expression pattern changes in a model of hypoxia, in which blood vessel formation is stimulated. These results suggest that CDKL-1 may mediate signalling for blood vessel formation, which is essential for recovery following cardiovascular failure. |
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Wellcome Poster 2013 Abstract
Cardiovascular disease (CVD) is the single largest cause of death in the UK. It accounts for around 200,000 deaths a year, mostly as a result of heart attack or stroke. Arterial blockage (or occlusion) is a major factor in CVD. Blockage may occur when a blood clot obstructs the flow of blood in an artery. Our project aims to identify novel genes involved in CVD. A Clinical study identified 82 genes with significant differences in their expression levels as a result of a heart attack. We are looking for the expression pattern of novel gene orthologues in the Zebrafish model organism using in-situ hybridisation. Any genes we locate to the cardiovascular system could play a part in the response to a heart attack, or may serve as future biological markers for heart disease. |
Wellcome Poster 2012 Abstract
Cardiovascular disease (CVD) is the single largest cause of death in the UK. It accounts for around 200,000 deaths a year, mostly as a result of heart disease or a stroke. Arterial blockage (or occlusion) is a major factor in CVD. Blockage may occur when a blood clot obstructs the flow of blood in an artery.